dual index primer pairs (New England Biolabs)
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New England Biolabs
dual index primer pairs
Dual Index Primer Pairs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/index+primer/NEBNext+Multi+Olig+IL+96+Dual+index+prim/bio_rxiv__64898__2026__05__08__723508-214-28-32
Average 97 stars, based on 2751 article reviews
Dual Index Primer Pairs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/index+primer/NEBNext+Multi+Olig+IL+96+Dual+index+prim/bio_rxiv__64898__2026__05__08__723508-214-28-32
Average 97 stars, based on 2751 article reviews
dual index primer pairs - by Bioz Stars,
2026-09
97/100 stars
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Amplification:Article Title: SKP1A bound to Polycomb-silenced genes mediates degradation of PRC2 and preconditions their activation. Article Snippet: Article SKP1A bound to Polycomb-silenced genes mediates degradation of PRC2 and preconditions their activation Article Title: Three-dimensional molecular cartography of human cerebral organoids revealed by double-barcoded spatial transcriptomics. Article Snippet: This mix has been PCR amplifyied (98 C, 30 s; 15 cycles: 98 C 10 s; 65 C 75 s; then 65 C 5min; 12 C hold), cleanned at 0.9X ratio with SPRIselect beads (Beckman Coulter B23318) and resuspended in 24mL of ddH2O. .. A second round of PCR amplification has been performed by mixing the 24 mL of adaptors linked cDNA’s complementary-probe strand with 25 mL 2X Q5 high fidelity ot start master mix, 0.5 mL of universal primer and 0.5 mL of Construct:Article Title: SKP1A bound to Polycomb-silenced genes mediates degradation of PRC2 and preconditions their activation. Article Snippet: Article SKP1A bound to Polycomb-silenced genes mediates degradation of PRC2 and preconditions their activation Next-Generation Sequencing:Article Title: SKP1A bound to Polycomb-silenced genes mediates degradation of PRC2 and preconditions their activation. Article Snippet: Article SKP1A bound to Polycomb-silenced genes mediates degradation of PRC2 and preconditions their activation Polymerase Chain Reaction:Article Title: Three-dimensional molecular cartography of human cerebral organoids revealed by double-barcoded spatial transcriptomics. Article Snippet: This mix has been PCR amplifyied (98 C, 30 s; 15 cycles: 98 C 10 s; 65 C 75 s; then 65 C 5min; 12 C hold), cleanned at 0.9X ratio with SPRIselect beads (Beckman Coulter B23318) and resuspended in 24mL of ddH2O. .. A second round of PCR amplification has been performed by mixing the 24 mL of adaptors linked cDNA’s complementary-probe strand with 25 mL 2X Q5 high fidelity ot start master mix, 0.5 mL of universal primer and 0.5 mL of Article Title: SETD1B-mediated broad H3K4me3 controls proper temporal patterns of gene expression critical for spermatid development Article Snippet: .. After washing, the beads were used to amplify libraries using 15 cycles of PCR with the universal primer and Article Title: A Quantitative Sequencing Method for 5-Formylcytosine in RNA Article Snippet: .. PCR: To a 200 μL PCR-tube were added 12.5 μL LongAmp Taq 2x Master mix, 0.625 μL SR Primer for Illumina, 0.625 μL Article Title: Enhancer-promoter compatibility is mediated by the promoter-proximal region Article Snippet: .. For each technical replicate 8 PCR reactions (each 200ng gDNA) were set up to amplify barcode sequences using S7 Fusion Polymerase kit (#MD-S7-500, Mobidiag) with following PCR conditions: 98°C for 30 s; 30 cycles of 98°C for 10 s, 68°C for 30 s and 72°C for 30 s and using NEBNext® Multiplex Oligos for Illumina®, Article Title: SETD1B-mediated broad H3K4me3 controls proper temporal patterns of gene expression critical for spermatid development. Article Snippet: .. After washing, the beads were used to amplify libraries using 15 cycles of PCR with the universal primer and Article Title: Evaluation of N 6 -methyldeoxyadenosine antibody-based genomic profiling in eukaryotes. Article Snippet: For cleanup, 90 μL (1.8×) of AMpure XP beads was added to each sample following the same procedure above butwith elution in 17 μL 10mMTris (pH 7.5) followedby quantificationwith theQubit ssDNAassay kit (Invitrogen Q10212). .. For PCR enrichment, 15 μL of each sample was transferred to a new strip tube and combined with 5 μL of Universal PCR primer, 5 μL of Concentration Assay:Article Title: Three-dimensional molecular cartography of human cerebral organoids revealed by double-barcoded spatial transcriptomics. Article Snippet: This mix has been PCR amplifyied (98 C, 30 s; 15 cycles: 98 C 10 s; 65 C 75 s; then 65 C 5min; 12 C hold), cleanned at 0.9X ratio with SPRIselect beads (Beckman Coulter B23318) and resuspended in 24mL of ddH2O. .. A second round of PCR amplification has been performed by mixing the 24 mL of adaptors linked cDNA’s complementary-probe strand with 25 mL 2X Q5 high fidelity ot start master mix, 0.5 mL of universal primer and 0.5 mL of Multiplex Assay:Article Title: Three-dimensional molecular cartography of human cerebral organoids revealed by double-barcoded spatial transcriptomics. Article Snippet: This mix has been PCR amplifyied (98 C, 30 s; 15 cycles: 98 C 10 s; 65 C 75 s; then 65 C 5min; 12 C hold), cleanned at 0.9X ratio with SPRIselect beads (Beckman Coulter B23318) and resuspended in 24mL of ddH2O. .. A second round of PCR amplification has been performed by mixing the 24 mL of adaptors linked cDNA’s complementary-probe strand with 25 mL 2X Q5 high fidelity ot start master mix, 0.5 mL of universal primer and 0.5 mL of Article Title: A Quantitative Sequencing Method for 5-Formylcytosine in RNA Article Snippet: .. PCR: To a 200 μL PCR-tube were added 12.5 μL LongAmp Taq 2x Master mix, 0.625 μL SR Primer for Illumina, 0.625 μL Article Title: Enhancer-promoter compatibility is mediated by the promoter-proximal region Article Snippet: .. For each technical replicate 8 PCR reactions (each 200ng gDNA) were set up to amplify barcode sequences using S7 Fusion Polymerase kit (#MD-S7-500, Mobidiag) with following PCR conditions: 98°C for 30 s; 30 cycles of 98°C for 10 s, 68°C for 30 s and 72°C for 30 s and using NEBNext® Multiplex Oligos for Illumina®, Article Title: Evaluation of N 6 -methyldeoxyadenosine antibody-based genomic profiling in eukaryotes. Article Snippet: For cleanup, 90 μL (1.8×) of AMpure XP beads was added to each sample following the same procedure above butwith elution in 17 μL 10mMTris (pH 7.5) followedby quantificationwith theQubit ssDNAassay kit (Invitrogen Q10212). .. For PCR enrichment, 15 μL of each sample was transferred to a new strip tube and combined with 5 μL of Universal PCR primer, 5 μL of other:Article Title: Protocol for the recovery and deep sequencing of short ssDNA pools from transient, fuel-dependent coacervate droplets. Article Snippet: Stripping Membranes:Article Title: Evaluation of N 6 -methyldeoxyadenosine antibody-based genomic profiling in eukaryotes. Article Snippet: For cleanup, 90 μL (1.8×) of AMpure XP beads was added to each sample following the same procedure above butwith elution in 17 μL 10mMTris (pH 7.5) followedby quantificationwith theQubit ssDNAassay kit (Invitrogen Q10212). .. For PCR enrichment, 15 μL of each sample was transferred to a new strip tube and combined with 5 μL of Universal PCR primer, 5 μL of Incubation:Article Title: Evaluation of N 6 -methyldeoxyadenosine antibody-based genomic profiling in eukaryotes. Article Snippet: For cleanup, 90 μL (1.8×) of AMpure XP beads was added to each sample following the same procedure above butwith elution in 17 μL 10mMTris (pH 7.5) followedby quantificationwith theQubit ssDNAassay kit (Invitrogen Q10212). .. For PCR enrichment, 15 μL of each sample was transferred to a new strip tube and combined with 5 μL of Universal PCR primer, 5 μL of |